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embryonic day-14 mouse striata neurospheres  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc embryonic day-14 mouse striata neurospheres
    (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of <t>neurospheres</t> in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.
    Embryonic Day 14 Mouse Striata Neurospheres, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryonic+day-14+mouse+striata+neurospheres/neurosphere+cells+isolated+from+murine+embryonic+day+14++e14++cerebral+cortices/pmc04394995-71-2-8
    Average 90 stars, based on 1 article reviews
    embryonic day-14 mouse striata neurospheres - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia"

    Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0122833

    (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of neurospheres in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.
    Figure Legend Snippet: (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of neurospheres in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.

    Techniques Used: Staining, Cell Culture, Positive Control

    Related Articles

    Staining:

    Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia
    Article Snippet: Embryonic day-14 mouse striata neurospheres were purchased from Stem Cell Technologies (Vancouver, Canada). .. T

    Cell Culture:

    Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia
    Article Snippet: Embryonic day-14 mouse striata neurospheres were purchased from Stem Cell Technologies (Vancouver, Canada). .. T

    Positive Control:

    Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia
    Article Snippet: Embryonic day-14 mouse striata neurospheres were purchased from Stem Cell Technologies (Vancouver, Canada). .. T



    Similar Products

    embryonic day-14 mouse striata neurospheres  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc embryonic day-14 mouse striata neurospheres
    (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of <t>neurospheres</t> in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.
    Embryonic Day 14 Mouse Striata Neurospheres, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryonic+day-14+mouse+striata+neurospheres/neurosphere+cells+isolated+from+murine+embryonic+day+14++e14++cerebral+cortices/pmc04394995-71-2-8
    Average 90 stars, based on 1 article reviews
    embryonic day-14 mouse striata neurospheres - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of neurospheres in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.

    Journal: PLoS ONE

    Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia

    doi: 10.1371/journal.pone.0122833

    Figure Lengend Snippet: (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of neurospheres in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.

    Article Snippet: Embryonic day-14 mouse striata neurospheres were purchased from Stem Cell Technologies (Vancouver, Canada).

    Techniques: Staining, Cell Culture, Positive Control